New import-substituting technology for obtaining cellular plasma therapy drugs

Vollständiger Abstract

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In recent decades, autologous cellular plasma therapy (ACPT) has become a promising method for treating musculoskeletal diseases due to the ability of activated blood cells to secrete growth factors, anti-inflammatory and analgesic cytokines. However, the high cost of foreign consumables and equipment limits the availability of therapy. Study of a new domestic technology for obtaining plasma preparations (L-PE) and its comparison with the foreign RegenLab (RL) technology in terms of production methods, activation methods and cellular composition of the resulting preparations. The study was conducted from March 2024 to May 2025 at the "Center for Traumatology and Orthopedics" of the N.A. Semashko Russian Railways - Medicine Clinical Hospital, which is the base for the Department of Traumatology, Orthopedics and Disaster Medicine of the Russian University of Medicine of the Ministry of Health of the Russian Federation, and at the Department of Pathological Physiology of the Russian University of Medicine of the Ministry of Health of the Russian Federation. The study involved 20 patients aged 25 to 58 years with diseases and injuries of the musculoskeletal system, from whom 10 ml of blood was taken from a vein. SPT preparations were prepared and studied using a new domestic technology - 12 patients, and using RL technology - 8 patients. L-PE contains platelets, leukocytes and erythrocytes, which expands the range of biologically active substances (BAS) compared to PRP, which includes only platelets. The study of the cellular composition showed that the domestic technology makes it possible to controllably obtain preparations with both high and low concentrations of formed elements. Activation with calcium chloride increases the release of growth factors, including hemolysis products and erythrocyte microvesicles, which enhances the regenerative potential. L-PE: cost-effective, easy to use, no mechanical damage to cells. However, further research is required to optimize clinical use.