MiR-150-5p suppresses high-grade transformation of lacrimal adenoid cystic carcinoma by downregulating COL1A1

Vollständiger Abstract

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Lacrimal adenoid cystic carcinoma (LACC) is the most common malignant epithelial tumor of the lacrimal gland, with particularly high recurrence and mortality in cases with high-grade transformation (HGT-LACC). This study aims to investigate the functional role of miR-150-5p in regulating the HGT of LACC. MiRNA microarray and proteomic mass spectrometry were performed on paired LACC and HGT-LACC tissues to identify differentially expressed miR-150-5p and its potential target COL1A1. The miR-150-5p/COL1A1 interaction was validated by luciferase reporter assay, and the effects of miR-150-5p on proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) were assessed in vitro and in vivo via colony formation, wound healing, Transwell assays, and xenograft models. MiR-150-5p was significantly downregulated in HGT-LACC and correlated with poor prognosis. Overexpression of miR-150-5p suppressed cell proliferation, migration and invasion, while its knockdown showed opposite effects. COL1A1 was confirmed as the direct target of miR-150-5p, and COL1A1 restoration counteracted the tumor-suppressive effect of miR-150-5p. In vivo , miR-150-5p overexpression inhibited tumor growth. Overall, our findings demonstrate that miR-150-5p suppresses LACC cell proliferation, migration, invasion, and EMT by downregulating COL1A1 expression, thereby functioning as a tumor suppressor. The miR-150-5p-COL1A1 axis provides novel insights into the pathogenesis of LACC and serves as a promising therapeutic target for LACC intervention.