Vollständiger Abstract
Worum geht es in dieser Arbeit?
This article aims to establish a novel cytochrome b real-time PCR assay using Taqman probe for identification of P. malariae and its discrimination from other Plasmodium human infecting species. The optimization of real-time PCR assay with 1X QuantiTect Probe PCR Master Mix, primers and probe used at concentrations of 0.4 μM and 0.1 μM, respectively; and 2.5 mM MgCl2, 5 μl DNA template and deionized H2O of 20 μl, was performed using a real-time PCR instrument. The developed real-time PCR assay was evaluated for the limit of detection, stability on standard panels (109-100 copies/ µl), as well as the sensitivity, specificity on control groups. The probit analysis demonstrates that the 95% detection limit was
Bibliografischer Nachweis
Publikationsdaten
- Autor:innen
- Dinh Thi Thu Hang, Vu Thi Nga, Hoang Van Tong, Hoang Xuan Su, Le Quoc Tuan, Nguyen Van Chuyen, Nguyen Thi Minh Trinh, Ho Anh Son
- Quelle
- VNU Journal of Science: Medical and Pharmaceutical Sciences
- Publikation
- 2020-01-01
- Band / Ausgabe
- Nicht angegeben
- Seiten
- Nicht angegeben
- ISSN / ISBN
- 2588-1132, 2615-9309
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Zitierfähiger Nachweis
Dinh Thi Thu Hang, Vu Thi Nga, Hoang Van Tong, Hoang Xuan Su, Le Quoc Tuan, Nguyen Van Chuyen, Nguyen Thi Minh Trinh, Ho Anh Son (2020). Development of a Novel Cytochrome b Real-Time PCR Assay for Identification of Plasmodium malariae. VNU Journal of Science: Medical and Pharmaceutical Sciences. https://doi.org/10.4269/ajtmh.26-0152