Vollständiger Abstract
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Abstract Whole exome sequencing is a technique that aims to selectively sequence all exons of protein-coding genes. A canine whole exome sequencing enrichment kit was designed based on the latest canine reference genome (build 3.1.72). Its performance was tested by sequencing 2 exome captures, each consisting of 4 pre-capture pooled, barcoded Illumina libraries on an Illumina HiSeq 2500. At an average sequencing depth of 102x, 83 to 86% of the target regions were completely sequenced with a minimum coverage of five and 90% of the reads mapped on the target regions. Additionally, it is shown that the reproducibility within and between captures is high and that pooling four samples per capture is a valid option. Overall, we have demonstrated the strong performance of this WES enrichment kit and are confident it will be a valuable tool in future disease association studies.
Bibliografischer Nachweis
Publikationsdaten
- Autor:innen
- Bart J. G. Broeckx, Frank Coopman, Geert E. C. Verhoeven, Valérie Bavegems, Sarah De Keulenaer, Ellen De Meester, Filip Van Niewerburgh, Dieter Deforce
- Quelle
- Scientific Reports
- Publikation
- 2014-01-01
- Band / Ausgabe
- Nicht angegeben
- Seiten
- Nicht angegeben
- ISSN / ISBN
- 2045-2322
- Zitationen
- 24 laut Crossref
- Referenzen
- 0 hinterlegt
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Zitierfähiger Nachweis
Bart J. G. Broeckx, Frank Coopman, Geert E. C. Verhoeven, Valérie Bavegems, Sarah De Keulenaer, Ellen De Meester, Filip Van Niewerburgh, Dieter Deforce (2014). Development and performance of a targeted whole exome sequencing enrichment kit for the dog (Canis Familiaris Build 3.1). Scientific Reports. https://doi.org/10.67815/ijcioes.3.1.72
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