ACS Nano
Living Microbe-Driven Bioorthogonal Catalysts Enables Cytotoxin Generation, Cuproptosis and Lactate Depletion for Synergistic Tumor Therapy
Abstract Bioorthogonal chemistry provides a compelling strategy for in situ cytotoxin generation. However, the clinical translation of conventional bioorthogonal catalysts is impeded by poor tumor accumulation, insufficient targeting specificity, limited in vivo catalytic efficiency, and inadequate spatiotemporal control. Meanwhile, excessive lactate accumulation in the tumor microenvironment (TME) drives metabolic reprogramming, malignant progression, and therapeutic resistance. Here, we develop a living bacterial …