One Health Journal
PCR-Based Detection of Priority Bacterial Biological Threat Agents: Advances in Diagnosis of Anthrax, Tularemia and Brucellosis
The rapid and accurate detection of high-consequence bacterial pathogens is essential for public health preparedness, veterinary medicine, food safety, and biodefense. Within the One Health framework, molecular diagnostics have become indispensable for integrated surveillance of zoonotic diseases affecting humans, animals, and the environment. This review summarizes current advances in polymerase chain reaction (PCR)-based detection of three priority bacterial biological threat agents: Bacillus anthracis, Francisel …